By M. W. Fowler, G. S. Warren, Murray Moo-Young
This present day it really is normally permitted that one of many key parts of biotechnology for the following century should be in plant-based biotechnology. Biotechnology has created new possibilities for plant scientists, with very important functions to agriculture and forestry. This reference textual content is split into 5 sections for ease of presentation. the 1st part specializes in the constitution, composition and performance of plant cells and genes with specific emphasis at the mobile and molecular biology of vegetation and cultured cells. part is worried with the direct exploitation of mobilephone cultures for the creation of invaluable elements. The 3rd part offers with regeneration and propagation platforms. The fourth part considers the more and more critical zone of genetic manipulation of plant cellphone platforms. The final part is on particular purposes in plant biotechnology. This reference paintings is a survey of those a number of features of plant biotechnology. the person chapters and the follow-up literature pointed out enable a simple entry to some of the topic parts and should, with a bit of luck, stimulate curiosity in those quickly relocating and interesting fields of analysis
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Additional resources for Plant Biotechnology. Comprehensive Biotechnology: Second Supplement
Sample text
The genes of Arabidopsis appear to be shorter than in other higher plants and there are obviously far fewer dispersed repetitive sequences in this very small genome. Taking the value of 75% of the total genome as comprising the unique fraction of the Arabidopsis genome suggests that the minimum information content required for a functional higher plant is 52 500 kb. Thus in all other higher plants shown in Table 1 the necessary information would comprise a much lower proportion of the total DNA.
One method is to use a two-element system, based on Ac-Ds. Here a Ds element, that is one that cannot transpose itself, but can be mobilized, is introduced into one line. Another Ds, which cannot be mobilized itself, but can mobilize other Ds elements is introduced into a second line. These two lines can be checked to ensure that the insertions are not on the same chromosome, although this is not an essential requirement. A hybrid between these two lines will contain an active transposable element system.
A second consideration is the status of the conventional genetic map in the species under investigation. In maize, the existence of a detailed genetic map with marker stocks has greatly facilitated the assignment of RFLP markers to specific chromosomes. However, in the absence of a preliminary map, the use of RFLPs should be the quickest and easiest method of generating the series of linkage groups, although it may not be possible to assign any one of these linkage groups to a specific chromosome.