By Julia E. Lever (auth.), Mary Taub (eds.)
Epithelial cells are found in many alternative tissues within the physique, and own a various variety of useful homes. even though, all epithelial cells percentage a few universal features. The cells own a morphological polarity (an-apical and basolateral surface), and are interconnected by means of tight junctions. The epithelial cells additionally own the skill to move decide on solutes around the monolayer. delivery structures localized on both the apical or basolateral floor are respon sible for this vectorial shipping. Such features of epithelial cells could be tested within the tissue tradition scenario. This quantity discusses using mobilephone tradition thoughts to check those basic homes of epithelial cells. Ma jor questions relating epithelia that could be tested in tradition are advert dressed. The methods that are taken to reply to those questions are defined intimately on the subject of kidney cellphone cultures. comparable investigations might be performed with epithelial mobile cultures derived from different tissues, following the kidney mobile tradition paradigm.
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Additional resources for Tissue Culture of Epithelial Cells
U, H. , Saier, M. , and Ellisman, M. , 1979, Tight junction formation is closely linked to the polar redistribution of intramembranous particles in aggregating MDCK epithelia, Exp. Cell Res. 122:384-392. U, H. , Saier, M. , 1980, Tight junction formation in the establishment of intramembranous particle polarity in aggregating MDCK cells, Exp. Cell Res. 128: 223-237. Valentich, J. , 1981, Morphological similarities between the dog kidney cell line MDCK and the mammalian cortical collecting tubule, Ann.
Lever, J. , 1982b, Expression of a differentiated transport function in apical membrane vesicles isolated from an established kidney epithelial cell line: Sodium electrochemical potential-mediated active sugar transport, J. Bioi. Chem. 257:8680-8686. Lever, J. , 1982c, Transepithelial ion transport and differentiation in epithelial cell cultures, in: Ions, Cell Proliferation and Cancer (A. L. Boynton, W. L. McKeehan, and J. F. ), Academic Press, New York, pp. 187-203. Lever,1. , and Sari, C. , 1983, Effect of tunicamycin on polarized membrane functions of an established kidney epithelial cell line, Biochim.
This model is based on that proposed to explain the cardiotonic actions of digitalis (Sweadner and Goldin, 1980). A Ca 2 + INa + antiport secondary active transport system in the plasma membrane catalyzes expulsion of Ca2 + from the cytoplasm coupled to Na + gradients maintained by Na + ,K + ATPase activity. Dissipation of transmembrane Na + gradients by inhibition of the Na + pump could result in increased levels of cytoplasmic Ca 2 + , which would presumably act as the signal for commitment to differentiation.