By Trevor A. Harper
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Additional resources for The Peripheral Blood Film
Vivax Bizarre forms. Band form stretching across red cell Like P. vivax Like P. vivax Multiple invasion is common. Small blue rings with single or double red chromatin dots. Some rings may lie along edge of cell (applique' form). Rings are smaller than those in P. vivax Not seen Deep blue signet ring with redchromatin dot. Size= 1/3 of red cell Blue, oval or round body occupying more than Vi red cell. Irregular outline. Scattered red dots Trophozoite, early Trophozoite, late No increase in cell size.
Falciparum which has no secondary exo-erythrocytic phase); some are phagocytosed by histiocytes. Other merozoites enter 53 Like P. vivax Bizarre forms. Band form stretching across red cell Like P. vivax Like P. vivax Multiple invasion is common. Small blue rings with single or double red chromatin dots. Some rings may lie along edge of cell (applique' form). Rings are smaller than those in P. vivax Not seen Deep blue signet ring with redchromatin dot. Size= 1/3 of red cell Blue, oval or round body occupying more than Vi red cell.
The granules (or lysosomes) are membrane-bound organelles, rich in hydrolytic enzymes. Primary granules contain peroxidase, acid phosphatase and other enzymes; secondary granules contain alkaline phosphatase and lysozyme (or muramidase). Tertiary granules contain acid phosphatase. Cytochemical reactions PMN are Feulgen positive. The PAS reaction reveals tightly packed magenta-coloured granules which do not obscure the nucleus; they are produced by the glycogen in the cytoplasm. 4 Cytochemical Enzyme Activity in Leucocytes and Plasma Cells Peroxidase Neutrophils +++ Eosinophils Basophils Monocytes +++ + Lymphocytes Plasma Cells — - Esterases Naphthyl Acetate — +++ Granular — - to ++ Phosphatases Chloroacetate +++ Granular — — — - Alkaline Acid - to +++ + to ++ — + to ++ +++ — - + to ++ +++ No activity, —; weak activity, +; moderate activity, ++; strong activity, +++ enzymes are used as markers for the neutrophilic series of cells.