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Cell lines are tested routinely for microbial contaminants including mycoplasma and species is verified by isoenzymology. Karyology, the DNA fingerprint for human lines, functional traits, tumorigenicity and other characteristics are confirmed as appropriate. Computerized databases on cell lines are now available internationally for ATCC clients via CODATA and TYMNET or Telenet networks. By using the databases one can quickly retrieve information about specific lines or create lists of lines with specific characteristics.

Pathol. 130, 472-484. M. D. (1985) 'Chaaracterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological and growth properties' Canc. Res. 45, 2924-2930. L. et ale (1990) 'Peripheral airway cell differentiation in human lung cancer cell lines' Canc. Res. 50, 5481-5487. J. J. (1990) 'Application of DNA fingerprints for cell line individualization'. Amer. J. Hum. Gen. 47, 499-514. , Krakauer, H. P. (1985a) 'Isolation and characterization of a monoclonal antibody specific for epithelial cells' Canc.

T. Tada, The University of Tokyo, Tokyo, Japan. 21 R. Sasaki and K. ), Animal Cell Culture and Production of Biologicals, 21-26. © 1991 Kluwer Academic Publishers. 11 of medium. The cells were cultured for 4 days, and serial dilutions of supernatant fluid of 13G2 cells were added to the mixture at the start ~f the culture. The cells were pulsed with 37 KBq of H-thY~idine during the last 20 h. The incorporation of H-thymidine was measured by standard liquid scintillation counting. Percent suppression was calculated by the formula: 100 x (l-(cpm of the culture wi th the supernatant) / (cpm of the culture without the supernatant) ) .

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