By Sherret S. Chase (auth.), S. K. Sen, Kenneth L. Giles (eds.)
The present and power significance of plant tissue tradition ideas in crop development is difficult to overemphasize. There are few components the place those thoughts could have extra attainable im pact than in tropical agriculture, the place the supply of excessive productiveness forms is unfortunately missing in lots of species. the possibility of the speedy, clonal propagation of elite participants and using managed multiline planting can have an important impact on crop yield and ailment resistance in lots of parts of the realm. This quantity is a suite of papers awarded on the Con ference on "Crop development via Tissue Culture", held on the Base Institute, Calcutta, India in December 1981. It makes an attempt to collect neighborhood study employees, acquainted with the agri cultural assets of the world and tissue tradition and molecular four point staff. It was once the wish of the convention that the "cross fertilization" of rules might result in new ways and job during this sector. The editors belief that this choice of papers will stimu~ past due curiosity and study within the tissue tradition and development of crop crops all over. v ACKNOWLEDGEMENTS The symposium from which the papers during this e-book are drawn was once held at Bose Institute, Calcutta on December 6 to December 10, 1981.
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Additional info for Plant Cell Culture in Crop Improvement
B: The vials containing precultured meristems are maintained in ice during treatment with the cryoprotectant. Fig. C: A container employed for immersing the vials in liquid nitrogen. Fig. D: A liquid nitrogen cylinder (VEB, German Democratic Republic) employed for storage. Fig. E: Induction of growth in a retrieved culture (a meristem precultured for one week followed by freezing). Figs. F-H: Plantlets obtained from meristems freeze-preserved for 14 months; these cultures were maintained in diffuse light.
Academic Press, New York (1976). T. Murashige and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant. 15:473-497 (1962).
J. Ashwood-Smith and J. Farrant, "Low Temperature Preservation in Medicine and Biology," Pitman Medicine, London (1980). S. Bajaj, Initiation of shoots and callus from potato tuber sprouts and axillary buds frozen at -l96°C, Crop Improv. 4:48-53 (1977b). S. Bajaj, Tuberization in potato plants regenerated from freeze-preserved meristems, Crop Improv. 5:137-141 (l978c). S. Bajaj, Regeneration of plants from potato meristems freeze-preserved for 24 months, Euphytica 30:141-146 (198lc). S. Bajaj, Clonal multiplication and cryopreservation of cassava through tissue culture, Crop Improv.