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By B. Q. Schillywink

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Compo Physiol. Biochem. Part C Primor N (1984) Pardaxin produces postjunctional muscle contraction in guineapig intestinal smooth muscle. Br J Pharmacol 82: 43-49 Reed LY, Meunch M (1983) A simple method for estimating fifty percent end points. Am J Hyg 27: 493-497 Schlieper P, De Robertis E (1977) Triton X-I00 as a channel forming substance in artificial lipid bilayer membrane. Arch Biochem Biophys 184: 207-208 Shin ML, Michaels DW, Mayer MM (1979) Membrane damage by a toxin from the sea anemone Stoichactis helianthus.

2). A test consisted of placing a shark in n, allowing it to adapt for at least 3 min and then giving "control trials" by injection of sea water into the shark's mouth five times. If the shark remained under n, a test trial consisting of injecting a known amount and concentration of test substance into the shark's buccal cavity was carried out. The injection was done by hand using a 5 cc syringe to which 3 mm diameter polyethylene tube was attached. The syringe contents were emptied in less than 1 s and emptying was timed to coincide with the start of an inhalation.

R. NELSON 3 1 Introduction The large rapaceous sharks are among the few wild animals that still pose a threat to the safety of humans. Any shark that attains a length of 2 m is potentially dangerous, but the majority of recorded shark attacks have been attributed to less than 50 species. Baldridge (1974) analysed approximately 1700 shark attacks and found that most were confmed to five families: The Lamnidae, Carcharhinidae, Orectolobidae, Odontaspidae, and Sphymidae. The same groups were implicated in South African attacks (Wallett 1978).

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