By E. Heftmann
Chromatography has emerged because the most vital and flexible analytical approach. The publication isn't just an up to date model of Heftmann's classical textual content, however it covers components of destiny value, equivalent to microfluidics and laptop assets. lower than his skilled counsel, gurus in every one box have contributed their useful event to an built-in remedy of recent micro research. partially A the theoretical foundation of person separation equipment is defined and the technical features are illustrated. It comprises the speculation of fuel and liquid chromatography in addition to particular chromatographic thoughts, corresponding to size-exclusion, planar, ion, and affinity chromatography in addition to numerous electrokinetic separation innovations. Microfluidics are lined for the 1st time and worthwhile resources of analytical tools are indexed and evaluated. 1. each one bankruptcy written via an authority2. Thorough therapy of the theoretical foundation of separation methods3. functional consultant for appearing analyses
Read or Download Chromatography, Sixth Edition: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and technique PDF
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Additional info for Chromatography, Sixth Edition: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and technique
Sample text
2 Parameter space . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Optimization methods . . . . . . . . . 1 Simultaneous methods . . . . . . . 2 Sequential methods . . . . . . . 3 Regression methods . . . . . . . 4 Theoretical methods .
1 Simultaneous methods . . . . . . . 2 Sequential methods . . . . . . . 3 Regression methods . . . . . . . 4 Theoretical methods . . . . . . . 4 Univariate optimization . . . . . . . . 1 Temperature . . . . . . . . . 2 Stationary-phase composition . . . . . 3 Mobile-phase composition . . . . . . 4 Other parameters . . . . . . . . 5 Multivariate optimization . . . . . . . . 1 Temperature and pressure . . . . . . 2 Temperature and flow-rate .
0 that reflects the packing structure. Hence, to a first approximation, the extent of broadening reflects only the particle diameter and the physical structure of the packed bed, but is independent of the linear velocity. 2 Longitudinal diffusion The random motion of molecules also leads to broadening of the solute zone. As discussed in Sec. 1, this broadening results in a symmetric Gaussian zone profile with a variance given by Eqn. 14. The extent of diffusion depends upon the diffusion coefficient and the time over which diffusion occurs.